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Yang, H., et al., Nrf1 and Nrf2 regulate rat glutamate-cysteine ligase catalytic subunit transcription indirectly via NF-kappaB and AP-1
Tissue sections were rehydrated in 1xPBS, blocked for non-specific binding in 5% BSA and 0.1% triton for 1-hour at 4 C, and then stained with primary conjugated antibodies for cell and nuclear shape analysis
Published research protocols rarely exceed 4 mg per day injected
All tests were done in isometric mode at the initial set voltage at which they generated maximum force in response to noradrenaline infusion (10 mol/L)
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